Abstract and Figures ABA Insensitive 5 (ABI5) is a basic leucine zipper transcription factor that plays a key role in the regulation of seed germination and early seedling growth in the presence of

Characterization of abi5 mutants in a second legume species, pea (Pisum sativum), confirmed a role for ABI5 in the regulation of longevity, seed degreening, and RFO accumulation, identifying ABI5 as a prominent regulator of late seed maturation in legumes.

Seedlings were further treated with or without 50 mM ABA for 30 min. HA-ABI5 was immunoprecipitated by using rabbit antibody to HA coupled to beads according to methods de- Since ABI5 is the direct target of FHY3 and FAR1, mutation of ABI5 also promotes seed germination in the presence of high concentrations of ABA (Finkelstein, 1994). In addition, we found that plants deficient in FHY3 or FAR1 are less sensitive to ABA -mediated stomatal movement than are wild-type plants; therefore, FHY3 and FAR1 confer increased resistance to drought ( Fig. 8 ). INTRODUCTION. The phytohormone abscisic acid is a major phytohormone that regulates plant growth and development, including seed dormancy, seed germination, seedling growth, and stomatal aperture, as well as plant responses to various abiotic and biotic stresses, such as drought, salt, and cold stresses and pathogen infection (Mauch-Mani and Mauch, 2005; Fujita et al., 2011; Hauser et al., 2011). Furthermore, we discovered that MED16 may possibly compete with MED25 to interact with the key transcription factor ABA INSENSITIVE 5 (ABI5) to positively regulate ABA signaling.

Abi5 review

ABA-induced flavonol biosynthesis involves HY5, ABI5, and PFG genes. • The flavonol quercetin regulates the ABA signaling network. • The mutual regulation of ABA and flavonol signaling is an ancestral trait of land plants. • The flavonol-phytohormone relationship is at the core of land plant evolution. The expression of ABI5 is upregulated by ABI3, and ABI3 itself can also directly regulate the expression of key negative regulators involved in the seed-to-seedling transition 42. These results Seed germination and subsequent seedling establishment are important developmental processes that undergo extremely complex changes of physiological status and are precisely regulated at transcriptional and translational levels. Phytohormones including abscisic acid (ABA) and gibberellin (GA) are th … Abi5 LLC is a Florida Domestic Limited-Liability Company filed On February 22, 2021.

ABA-induced flavonol biosynthesis involves HY5, ABI5, and PFG genes. The flavonol quercetin regulates the ABA signaling network.

The basic Leucine zipper transcription factor ABSCISIC ACID INSENSITIVE5 (ABI5) is a key regulator of abscisic acid ()–mediated seed germination and postgermination seedling growth.While a family of SUCROSE NONFERMENTING1-related protein kinase2s (SnRK2s) is responsible for ABA-induced phosphorylation and stabilization of ABI5, the phosphatase(s) responsible for dephosphorylating ABI5 …

Therefore, our study uncovers a new role of ABI5 in vegetative development in plants, and implies a role of ABA signaling in vegetative development in Arabidopsis. 2016-11-01 The target genes of ABI5 enable further adaptations to abiotic stresses. In this review, we present the current understanding of the way ABI5 functions as (1) a regulator of abiotic stress responses and (2) an integrator of ABA crosstalk with other phytohormones.

2020-03-24

Although the mechanisms by which the expression of ABI5 is regulated by ABI3 are unknown, a key observation of our work is that ABI5 can complement abi3‐1, whereas ABI3 cannot complement abi5‐4. This strongly suggests that ABI5 acts downstream of ABI3 to arrest growth of germinated embryos by ABA. Disruption of ABI5 increases Arabidopsis ABA‐insensitivity and decreases the expression of many ABA‐responsive genes (Finkelstein and Lynch, 2000b), whereas ABI5‐overexpressing plants were hypersensitive to ABA during seed germination and early seedling development (Lopez‐Molina et al., 2001). WTy35S::HA-ABI5 transgenic seedlings (T3 generation) were treated in liquid MS medium with [32P]orthophosphoric acid [100 mCi (1 Ci 5 37 GBq), 285.5 Ciymg, NEN] for 90 min. Seedlings were further treated with or without 50 mM ABA for 30 min. HA-ABI5 was immunoprecipitated by using rabbit antibody to HA coupled to beads according to methods de- Since ABI5 is the direct target of FHY3 and FAR1, mutation of ABI5 also promotes seed germination in the presence of high concentrations of ABA (Finkelstein, 1994).

Consistently, med16 and med25 mutants displayed opposite phenotypes in ABA response, cuticle permeability, and differential ABI5‐mediated EM1 and EM6 expression.
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ABI5 is the best-studied key molecule in the ABA signaling pathway with respect to PTMs; therefore, this review could serve as a model to guide post-translational studies of important regulators in other plant hormone signaling pathways. Keywords: (de-)phosphorylation; ABA signaling; ABI5; sumoylation; ubiquitination. As a crucial regulator of ABA signaling, ABSCISIC ACID-INSENSITIVE5 (ABI5) is involved in many aspects of plant growth and development, yet its regulation of anthocyanin biosynthesis has not been elucidated. In this study, we found that MdABI5, the apple homolog of Arabidopsis ABI5, positively regulated ABA-induced anthocyanin biosynthesis.

Furthermore, we discovered that MED16 may possibly compete with MED25 to interact with the key transcription factor ABA INSENSITIVE 5 (ABI5) to positively regulate ABA signaling.
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The abi5 mutant (SALK_013163; Zheng et al., 2012) was obtained from the ABRC, and the T‐DNA insertion is approximately 1 kb upstream of the ABI5 transcription start site. qRT‐PCR analyses showed that the transcription levels of ABI5 and RAV1 were markedly decreased in the RAV1‐U abi5 double mutant (Figure S6).

Abi5 LLC is a Florida Domestic Limited-Liability Company filed On February 22, 2021. The company's filing status is listed as Active and its File Number is L21000090327. The Registered Agent on file for this company is AB Asset Management LLC and is located at 301 Arthur Godfrey Road, Miami Beach, FL 33140.

To generate fine-mapping populations with closely linked recombinations, we out-crossed abi5-1 and abi5-3 (in the Wassilewskija and Colum-bia [Col] backgrounds, respectively) to lines carrying Sumoylation of ABI5 by the ArabidopsisSUMO E3 ligase SIZ1 negatively regulates abscisic acid signaling Kenji Miuraa,b,1, Jiyoung Leec,2, Jing Bo Jina,3, Chan Yul Yooa, Tomoko Miuraa, and Paul M. Hasegawaa,1 aCenter for Plant Environmental Stress Physiology, Purdue University, West Lafayette, IN 47907; bGraduate School of Life and Environmental Sciences, 2016-01-04 2001-04-10 Participates in ABA-regulated gene expression during seed development and subsequent vegetative stage by acting as the major mediator of ABA repression of growth. Binds to the embryo specification element and the ABA-responsive element (ABRE) of the Dc3 gene promoter and to the ABRE of the Em1 and Em6 genes promoters. Can also trans-activate its own promoter, suggesting that it is autoregulated. 2014-11-01 Consistently, med16 and med25 mutants displayed opposite phenotypes in ABA response, cuticle permeability, and differential ABI5‐mediated EM1 and EM6 expression. Together, our data indicate that MED16 and MED25 differentially regulate ABA signaling by antagonistically affecting ABI5‐mediated transcription in Arabidopsis. Regulation and Role of the Arabidopsis Abscisic Acid-Insensitive 5 Gene in Abscisic Acid, Sugar, and Stress Response1 Ine`s M. Brocard, Tim J. Lynch, and Ruth R. Finkelstein* Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, 2000-04-01 2009-03-31 Background ABI5 (abscisic acid insensitive 5) is involved in ABA-regulated gene expression during seed development and subsequent vegetative stage and acts as the major mediator of ABA repression of growth. Binds to the embryo specification element and the ABA-responsive element (ABRE) of the Dc3 gene promoter and to the ABRE of the Em1 and Em6 genes promoters.